BioVision Inc Caspase-3 Colorimetric Assay Kit: Caspase-3/CPP32 Activity Colorimetric Assay Kit: Simple & Convenient Colorimetric Assay to Measure Caspase-3/CPP32 Activity within 1-2 hrs. Kit Size: 25, 100, 200 and 400 assays.
Manufacturer: BioVision Inc K10625
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-3/CPP32 Colorimetric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD. The assay is based on spectrophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate DEVD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in CPP32 activity. BioVision charges an Ice pack and Insulated Container fee of $25.00 for each order.
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