BioVision Inc Caspase-3 Fluorometric Assay Kit: Caspase-3/CPP32 Activity Fluorometric Assay Kit: Simple & Convenient Fluorometric Assay to Measure Caspase-3/CPP32 Activity within 1-2 hrs. Kit Size: 25, 100, 200 and 400 assays.
Manufacturer: BioVision Inc K10525
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The CPP32/Caspase-3 Fluorometric Protease Assay Kit provides a simple and convenient means for assaying the DEVD-dependent caspase activity. The assay is based on detection of cleavage of substrate DEVD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). DEVD-AFC emits blue light (λmax = 400 nm); upon cleavage of the substrate by CPP32 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-3/CPP32 activity. BioVision charges an Ice pack and Insulated Container fee of $25.00 for each order.
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