BioVision Inc Cathepsin H Activity Fluorometric Assay Kit: Cathepsin-H Activity Assay Kit: Rapid & Convenient Fluorometric Assay to Measure Cathepsin-H Activity within 1-2 hrs. 100 Assays.
Manufacturer: BioVision Inc K145100
Apoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations. The Cathepsin-H Activity Assay kit is a fluorescence-based assay that utilizes the preferred cathepsin-H substrate Arginine labeled with AFC (amino-4-trifluoromethyl coumarin). Cell lysates or other samples that contain cathepsin-H will cleave the synthetic substrate R-AFC to release free AFC. The released AFC can easily be quantified using a fluorometer or fluorescence plate reader. BioVision charges an Ice pack and Insulated Container fee of $25.00 for each order.
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