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Novus Biologicals™ CCL2/JE/MCP-1 Antibody Pair [HRP]

Rabbit Monoclonal Antibody Pair

Manufacturer:  Novus Biologicals™ NBP27944815PLATES

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Catalog No. NB009935


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Description

Description

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human CCL2/JE/MCP-1.
Specifications

Specifications

HRP
Antibody Pairs
2.813 pg/mL
6347
RUO
CCL2/JE/MCP-1
15 Plates
2.813-180 pg/mL
C-C motif chemokine ligand 2, CCL2, GDCF-2, HC11, HSMCR30, MCAF, Mcp1, Mcp-1, SCYA2, SMC-CF
Sandwich ELISA
This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human CCL2/JE/MCP-1.
Sandwich ELISA
  • Standard: Each vial contains 12 ng of recombinant Human CCL2/JE/MCP-1.
  • Capture Antibody: 1 mg/mL of rabbit anti-Human CCL2/JE/MCP-1 monoclonal antibody (in PBS, pH 7.4).
  • Detection Antibody: 0.2 mg/mL of rabbit anti-Human CCL2/JE/MCP-1 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
  • Storage is content dependent.
    Rabbit
    Human
    CCL2
    1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 180 pg/mL is recommended.
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