CD103 (Integrin alpha E) Monoclonal Antibody (2E7), Biotin, eBioscience™, Invitrogen™

Armenian Hamster Monoclonal Antibody

Supplier:  Invitrogen 13103185

Catalog No. 50-116-88

Description

Description: The 2E7 monoclonal antibody reacts with mouse CD103, the alphaE integrin. CD103 non-covalently associates with integrin beta7 and is expressed on all intraepithelial lymphocytes, a small subset of peripheral lymphocytes, and dendritic epidermal T cells (DEC). Epithelial cell antigen E-cadherin binds to CD103 and mediates homing of lymphocytes to the intestinal epithelium. Applications Reported: This 2E7 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections. Applications Tested: The 2E7 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 μm post-manufacturing filtered.

CD103 (ITGAE, Integrin alpha E) is molecule that is expressed on mucosa-associated T lymphocytes and activated cells and on subet of TGF beta -1 cells. In particular, CD103 includes a 150kDa alpha chain and a 120kDa beta chain. CD103 is expressed by 0.5 to 2% of resting lymphocytes in peripheral blood and lymphoid organs. CD103 antibody stains a few bone marrow cells and, rarely, peripheral blood lymphocytes. CD103 is part of the integrin family which are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. ITGAE encodes an I-domain-containing alpha integrin that undergoes post-translational cleavage in the extracellular domain, yielding disulfide-linked heavy and light chains. In combination with the beta 7 integrin, CD103 protein forms the E-cadherin binding integrin known as the human mucosal lymphocyte-1 antigen. CD103 is preferentially expressed in human intestinal intraepithelial lymphocytes (IEL), and in addition to a role in adhesion, it may serve as an accessory molecule for IEL activation. Diseases associated with CD103 dysfunction include hairy cell leukemia and splenic marginal zone lymphoma.

Specifications

• Antigen: CD103 (Integrin alpha E)
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q60677
• Gene Symbols: Itgae
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 16407
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry, Immunohistochemistry (Frozen)
• Clone: 2E7
• Conjugate: Biotin
• Gene: Itgae
• Gene Alias: A530055J10; alpha-E1; alpha-M290; aM290; antigen CD103; antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide; CD103; DC; DCs; HML-1 antigen; human mucosal lymphocyte antigen 1, alpha polypeptide; HUMINAE; integrin alpha E; integrin alpha E, epithelial-associated; integrin alpha E1, epithelial-associated; integrin alpha M290; Integrin alpha-E; Integrin alpha-E heavy chain; Integrin alpha-E light chain; Integrin alpha-IEL; integrin subunit alpha E; integrin, alpha E; integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide); integrin, alpha E, epithelial-associated; Intregrin alpha E2; Itgae; MGC141996; Mucosal lymphocyte 1 antigen
• Host Species: Armenian Hamster
• Quantity: 500 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG

For Research Use Only.