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CD103 (Integrin alpha E) Armenian Hamster anti-Mouse, eFluor® 450, Clone: 2E7, eBioscience™ Non-distribution product as customer accommodation.

Armenian Hamster Monoclonal Antibody

Manufacturer:  Invitrogen 48103182

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Catalog No. 50-112-4129


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Description

Description

Description: The 2E7 monoclonal antibody reacts with mouse CD103, the alphaE integrin. CD103 non-covalently associates with integrin beta7 and is expressed on all intraepithelial lymphocytes, a small subset of peripheral lymphocytes, and dendritic epidermal T cells (DEC). Epithelial cell antigen E-cadherin binds to CD103 and mediates homing of lymphocytes to the intestinal epithelium. Applications Reported: This 2E7 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 2E7 antibody has been tested by flow cytometric analysis of mouse lymph node cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser. Filtration: 0.2 µm post-manufacturing filtered.

The CD103 antigen is a 160-130-105 kDa molecule which is expressed on mucosa-associated T lymphocytes and activated cells and on subet of TGF beta -1 cells. This human intra epithelial lymphocyte marker is the E integrin chain associated with the b7 subunit. CD103 antibody stains a few bone marrow cells and, rarely, peripheral blood lymphocytes. CD103 is expressed by 0.5 to 2% of resting lymphocytes in peripheral blood and lymphoid organs.
Specifications

Specifications

CD103 (Integrin alpha E)
Monoclonal
0.2 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Q60677
ITGAE, HUMINAE
Armenian Hamster
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Flow Cytometry
2E7
eFluor™ 450
Itgae
Liquid
Itgae
IgG
100 μg
4° C, store in dark, DO NOT FREEZE!
Primary
16407
Documents
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