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CD106 (VCAM-1) Mouse anti-Human, PE-Cyanine7, Clone: STA, eBioscience™ Non-distribution product as customer accommodation.

Mouse Monoclonal Antibody

Manufacturer:  Invitrogen 25106942

Catalog No. 50-112-3322


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Description

Description

Description: The STA monoclonal antibody reacts with human CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1), a 110 kDa transmembrane glycoprotein expressed by myeloid lineage and bone marrow stromal cells. Endothelial cells constitutively express low levels of CD106 and upregulate it upon cytokine stimulation. CD106 binds to Integrin alpha4beta1 (VLA-4, CD49d/CD29) and Integrin alpha4beta7 (LPAM-1). Cytokine-mediated upregulation of CD106 on endothelial cells suggests a role for this antigen in the inflammatory response. Applications Reported: This STA antibody has been reported for use in flow cytometric analysis. Applications Tested: This STA antibody has been pre-titrated and tested by flow cytometric analysis of stimulated HUVEC. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation.

Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered. This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Two alternatively spliced transcripts encoding different isoforms have been described for this gene.
Specifications

Specifications

CD106 (VCAM-1)
Monoclonal
5 μL/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P19320
CD106, DKFZp779G2333, INCAM-100, MGC99561, CD106 antigen, vascular cell adhesion protein 1
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
STA
PE-Cyanine7
VCAM1
Liquid
VCAM1
IgG1, kappa
100 Tests
4° C, store in dark, DO NOT FREEZE!
Primary
7412
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