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CD107a (LAMP-1) Rat anti-Mouse, eFluor® 450, Clone: eBio1D4B (1D4B), eBioscience™ Non-distribution product as customer accommodation.

Rat Monoclonal Antibody

Manufacturer:  Invitrogen 48107182

Catalog No. 50-112-4134


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Description

Description

Description: The eBio1D4B monoclonal antibody reacts with mouse CD107a, also known as lysosomal-associated membrane protein-1 (LAMP-1). CD107a is a type I, lysosomal membrane protein that is extensively glycosylated. It is expressed constitutively in the late endosomes-lysosomes in all cells. CD107a is also transiently expressed on the cell surface of degranulating cytolytic T cells. Additionally, CD107a has been implicated in a variety of cellular functions including cancer metastasis and is also a marker for lysosomal storage disorders. Applications Reported: This 1D4B antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This 1D4B antibody has been tested by intracellular staining and flow cytometric analysis of mouse thioglycolate-elicited peritoneal exudate cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser. Filtration: 0.2 µm post-manufacturing filtered. CD107a (lysosome-associated membrane protein-1, LAMP-1), together with LAMP-2, is a major constituent of lysosomal membrane, 1-2% of total CD107a is found also on the plasma membrane. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes. Increased CD107a immunoreactivity is observed in neurones, and in glial cells surrounding senile plaques in Alzheimers disease cases and is localized mainly in medullary epithelial cells, single macrophages and lymphocytes in acute thymic involution. CD107a is a good marker of mast cell activation.
Specifications

Specifications

CD107a (LAMP-1)
Monoclonal
0.2 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P11438
CD107a, LAMPA, LGP120, CD107 antigen-like family member A, LAMP-1, lysosome-associated membrane glycoprotein 1, lysosome-associated membrane protein 1
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Flow Cytometry
eBio1D4B (1D4B)
eFluor™ 450
Lamp1
Liquid
Lamp1
IgG2a, kappa
100 μg
4° C, store in dark, DO NOT FREEZE!
Primary
16783
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