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CD107b (LAMP-2) Mouse anti-Human, Clone: eBioH4B4 (H4B4), eBioscience™ Non-distribution product as customer accommodation.

Mouse Monoclonal Antibody

$63.00 - $118.00

Specifications

Antigen CD107b (LAMP-2)
Clone eBioH4B4 (H4B4)
Target Species Human
Conjugate Unconjugated
Isotype IgG1, kappa
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Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
50-128-16 Non-distribution product as customer accommodation.
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Invitrogen
14107880
25 μg
Each for $63.00
Add to cart
 
50-128-17 Non-distribution product as customer accommodation.
View Documents
Invitrogen
14107882
100 μg
Each for $118.00
Add to cart
 
Description

Description

Description: The eBioH4B4 monoclonal antibody reacts with human CD107b, also known as lysosomal-associated membrane protein-2 (LAMP-2). CD107b is a highly glycosylated, type I transmembrane protein of approximately 105 kDa. It is expressed intracellularly in lysosomal/endosomal membranes in nearly all cells. It is also expressed on the surface of degranulating T cells (to a lesser extent than CD107a) and activated platelets as well as some cancer cells. In humans, mutations in CD107b results in a lysosomal glycogen storage disorder, known as Danon disease. Applications Reported: Purified anti-human CD107b (LAMP-2) has been reported for use in flow cytometric analysis. It has also been reported for use in surface staining in a flow cytometric based degranulation assay. (Fluorochrome conjugated eBioH4B4 (H4B4) is recommended for use in flow cytometry.). Applications Tested: This eBioH4B4 (H4B4) antibody has been tested by intracellular staining and flow cytometric analysis of Jurkat cell line. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This antibody has also been tested by immunoblotting at 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered. Lysosomes are membrane-bound vacuoles which play a critical role in cellular metabolism. These organelles participate in endocytosis, the synthesis and assembly of acid hydrolases, act as sites for digestion of foreign materials and for specialized autolytic cellular processes. Membrane-associated glycoproteins have an important role in normal lysosomal function. Prior to posttranslational modification, Lysosome Associated Membrane Protein 2 (LAMP 2) is an ~45 kDa polypeptide. Mature, functional LAMP 2 is extensively glycosylated with a variety of different N-linked and O-linked oligosaccharides with a total molecular weight of ~100-110 kDa. LAMP 2 has a structure comprised of a large amino-terminal intra-lysosomal domain, a hydrophobic trans-membrane domain, and a short carboxyl-terminal cytoplasmic tail.
Specifications

Specifications

CD107b (LAMP-2)
Human
IgG1, kappa
Monoclonal
Liquid
Antibody
4° C
eBioH4B4 (H4B4)
Unconjugated
Flow Cytometry, Western Blot
Primary
Monoclonal
PBS with 0.09% sodium azide; pH 7.2
RUO
SDS

For Research Use Only.

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