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CD108 Mouse anti-Human, Brilliant Violet 510, Clone: KS-2, BD Optibuild™

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 744579

Catalog No. BDB744579

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The KS-2 monoclonal antibody specifically recognizes the John-Milton-Hagen (JMH) blood group antigen. CD108 is a 76-80 kDa glycosylphosphatidylinositol (GPI)-linked protein. It is expressed on erythrocytes, at low levels on lymphocytes, but higher levels are detected on lymphoblasts and lymphoblastic cell lines. An adhesion function for CD108 has been suggested but this has not been demonstrated. Autoantibodies to this molecule are not uncommon and often associated with the loss of JMH antigen expression on erythrocytes.

The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 405nm and Em Max at 510nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50nm) filter set. BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser. BV510 is a polymer-based dye that is brighter than BD Horizon V500. Due to similar excitation and emission properties, BD Horizon BV510 and BD Horizon V500 cannot be used simultaneously.


Aqueous buffered solution containing ≤0.09% sodium azide.
JMH, SEM7A, Sema K1, Sema L, John-Milton-Hargen human blood group Ag
Mouse IgG2a, κ
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Brilliant Violet 510
Affinity Purified
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV510 under optimal conditions that minimize unconjugated dye and antibody.
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