CD117 (c-Kit) Monoclonal Antibody (2B8), Super Bright™ 436, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
62-117-182	Invitrogen 62117182	100 μg

Description

Description: The 2B8 monoclonal antibody reacts with mouse CD117, also known as c-Kit receptor, Steel factor receptor, and stem cell factor receptor. A member of the tyrosine kinase receptor family, this 145-kDa molecule is expressed by a majority of hematopoietic progenitor cells characterized in the mouse bone marrow as a small subset of cells positive for Sca-1 and Thy1 (Thy1 low) and negative for lineage markers. The interaction of the mouse c-Kit receptor and steel factor promotes the proliferation and differentiation of hematopoietic progenitor cells. CD117 is also expressed by mast cells and plays a role in signaling and activation of these cells. Applications Reported: This 2B8 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 2B8 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.06 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.

CD117 (c-Kit) Monoclonal antibody specifically detects CD117 (c-Kit) in Mouse, Porcine samples. It is validated for Flow Cytometry.

Specifications

• Antigen: CD117 (c-Kit)
• Concentration: 0.2 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Gene ID (Entrez): 16590, 396810
• Isotype: IgG2b κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

• Clone: 2B8
• Applications: Flow Cytometry
• Conjugate: Super Bright 436
• Host Species: Rat
• Target Species: Mouse, Porcine
• Gene Symbols: KIT
• Primary or Secondary: Primary
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!

For Research Use Only.