CD11b Monoclonal Antibody (ICRF44), Super Bright™ 436, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
62011842	Invitrogen 62011842	100 Tests

Description

Description: The ICRF44 monoclonal antibody reacts with human CD11b, a 165 kDa adhesion molecule. CD11b associated with integrin beta2 (CD18) is expressed on the surface of monocytes, granulocytes, activated lymphocytes and a subset of NK cells. CD11b is a receptor for intercellular adhesion molecule family members CD54, CD102 and CD50 as well as for iC3b. These adhesions are crucial in cell-cell and cell-matrix interactions. The ICRF44 monoclonal antibody crossreacts with baboon, chimpanzee, cynomologous monkey and Rhesus macaque. Applications Reported: This ICRF44 antibody has been reported for use in flow cytometric analysis. Applications Tested: This ICRF44 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10⁵ to 10⁸ cells/test. Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer

CD11b Monoclonal antibody specifically detects CD11b in Baboon, Chimpanzee, Cynomolgus Monkey, Human, Rhesus Monkey samples. It is validated for Flow Cytometry.

Specifications

• Antigen: CD11b
• Concentration: 5 μL/Test
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Gene Accession No.: P11215
• Isotype: IgG1 κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

• Clone: ICRF44
• Applications: Flow Cytometry
• Conjugate: Super Bright 436
• Host Species: Mouse
• Target Species: Baboon, Chimpanzee, Cynomolgus Monkey, Human, Rhesus Monkey
• Gene Symbols: ITGAM
• Primary or Secondary: Primary
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Gene: ITGAM

For Research Use Only