CD11c Mouse anti-Canine, Cynomolgus Monkey, Human, Non-human primate, APC-eFluor® 780, Clone: BU15, eBioscience
Mouse Monoclonal Antibody
Manufacturer: Invitrogen 47012841
Description: The BU15 monoclonal antibody reacts with canine, human, and non-human primate CD11c, the 150 kDa integrin aX chain. CD11c non-covalently associates with beta2 integrin to form the CD11c/CD18 heterodimer. This complex is expressed on monocytes, granulocytes, macrophages, NK, dendritic cells, and subset of T and B lymphocytes. CD11c/CD18 binds to CD54, iC3b and fibrinogen and plays a role in leukocyte adhesive interactions. CD11c also binds complement fragment iC3b to mediate phagocytosis of opsonized particles. The BU15 antibody has been reported to crossreact to cynomologus monkey. Moreover, this antibody has been reported to exhibit blocking activity. Crossblocking studies suggest that BU15 recognizes a different epitope from clone 3.9. Applications Reported: This BU15 antibody has been reported for use in flow cytometric analysis. Applications Tested: This BU15 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered. CD11, along with CD18, form a heterodimer adhesion molecule. In particular, CD11 is composed of CD11a, CD11b and CD11c. CD11a is a leukocyte marker that is expressed in B and T lymphocytes, macrophages, monocytes, neutrophils, basophils and eosinophils. CD11b is primarily expressed by monocytes, macrophages, natural killer cells, some B and T-cells, and granulocytes. CD11c is expressed in monocytes, macrophages, natural killer cells, some granulocytes and less so in a subset of lymphocytes.
|PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2|
|AI449405, Cd11c, Cr4, N418, CD11 antigen-like family member C, ITGAX, integrin aX, integrin alpha-X|
|Canine, Cynomolgus Monkey, Human, Non-human Primate|
|4° C, store in dark, DO NOT FREEZE!|
|102147272, 3687, 489929|
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