CD122 Rat anti-Mouse, PE, Clone: 5H4, eBioscience™
Rat Monoclonal Antibody
Manufacturer: Invitrogen 12122182
Description: The 5H4 antibody reacts with mouse CD122, the 90-110 kDa interleukin-2 receptor beta chain. CD122 is expressed by NK cells and some T cells in the periphery and is upregulated by activation. CD122 associates with CD25 and CD132 (common gamma chain) to form the high affinity IL-2 receptor. Binding of IL-2 to CD122 is not inhibited by the 5H4 antibody. Applications Reported: The 5H4 antibody has been reported for use in flow cytometric analysis. Applications Tested: The 5H4 antibody has been tested by flow cytometric analysis of resting and activated mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.CD122 (Interleukin 2 receptor) is involved in T cell-mediated immune responses, primarily expressed in the hematopoietic system and is present in 3 forms. The low affinity form of CD122 is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. Both the intermediate and high affinity forms of the receptor are involved in receptor-mediated endocytosis and transduction of mitogenic signals from CD122. The protein encoded by the CD122 gene represents the beta subunit and is a type I membrane protein. Diseases associate with CD122 protein dysfunction include oligoarticular juvenile idiopathic arthritis and rheumatoid factor negative juvenile idiopathic arthritis.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|4° C, store in dark, DO NOT FREEZE!|
|CD122, IL15RB, P70-75, CD122 antigen, IL-2 receptor subunit beta, IL-2R subunit beta, IL-2RB, high affinity IL-2 receptor beta subunit, high affinity IL-2 receptor subunit beta, interleukin 15 receptor, beta, interleukin-2 receptor subunit beta, p75|
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