CD123 Monoclonal Antibody (5B11), APC, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Supplier:  Invitrogen 17123182

Catalog No. 50-150-64

Description

Description: The 5B11 monoclonal antibody reacts with mouse CD123, the alpha chain of the IL-3 receptor. This 60-70 kDa transmembrane protein binds to IL-3 with low affinity by itself and when associated with either CD131 (common beta chain) or AIC2A (IL-3beta) binds IL-3 with high affinity. CD123 does not transduce any intracellular signals upon binding IL-3 and requires the beta chain for this function. Applications Reported: This 5B11 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 5B11 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.

CD123 Monoclonal antibody specifically detects CD123 in Mouse samples. It is validated for Flow Cytometry.

Specifications

• Antigen: CD123
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P26952
• Gene Symbols: IL3RA
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 16188
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: 5B11
• Conjugate: APC
• Gene: IL3RA
• Gene Alias: CD123; CD123 antigen; CDw123; Cyrl; cytokine receptor-like protein CYRL; hIL-3Ra; Il-3 alpha subunit; IL-3 receptor alpha chain; IL-3 receptor alpha SP2 isoform; IL-3 receptor subunit alpha; IL3R; IL-3R subunit alpha; Il3ra; IL-3RA; IL-3R-alpha; IL3RAY; IL3RX; IL3RY; interleukin 3 receptor subunit alpha; interleukin 3 receptor, alpha; interleukin 3 receptor, alpha (low affinity); interleukin 3 receptor, alpha chain; interleukin-3 receptor class II alpha chain; Interleukin-3 receptor subunit alpha; Sut-1
• Host Species: Rat
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2a κ

For Research Use Only.