Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

CD126 Rat anti-Mouse, PE-Cyanine7, Clone: D7715A7, eBioscience™ Non-distribution product as customer accommodation.

Rat Monoclonal Antibody

Manufacturer:  Invitrogen 25126182

 View more versions of this product

Catalog No. 50-112-3327


Add to cart

Description

Description

Description: The monoclonal antibody D7715A7 recognizes mouse CD126, the IL-6 receptor alpha chain, a member of the Ig superfamily. CD126 can heterodimerize with CD130 (gp130) to form the high affinity IL-6 receptor. Once IL-6 is bound, signaling from the CD130 subunit is initiated and the receptor complex is internalized. The IL-6 receptor is also reported to exist as a soluble version, which bound to IL-6 acts in trans to signal. Expression is found on T lymphocytes in the periphery and during development in the thymus is restricted to SP populations, unlike gp130 which is expressed in all thymocytes. Additional expression has been shown on activated B lymphocytes, macrophages, granulocytes and CD34+ hematopoietic progenitor cells. The monoclonal antibody D7715A7 binding can be blocked addition of ligand (IL-6). Applications Reported: This D7715A7 antibody has been reported for use in flow cytometric analysis. Applications Tested: This D7715A7 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive to photo- induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered. Interleukin 6 (IL6) is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an important role in immune response. The protein encoded by this gene is a subunit of the receptor complex for IL6. The IL6 receptor is a protein complex consisting of this protein and interleukin 6 signal transducer (IL6ST/GP130/IL6-beta), a receptor subunit also shared by many other cytokines. Dysregulated production of IL6 and this receptor are implicated in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
Specifications

Specifications

CD126
Monoclonal
0.2 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P22272
CD126, IL-6R-1, IL-6R-alpha, IL6RA, MGC104991, IL6R
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Flow Cytometry
D7715A7
PE-Cyanine7
Il6ra
Liquid
Il6ra
IgG2b
100 μg
4° C, store in dark, DO NOT FREEZE!
Primary
16194
Documents
Provide Content Correction

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit

For Research Use Only.