CD127 Rat anti-Mouse, Brilliant Violet 421, Clone: SB/199, BD
Rat Monoclonal Antibody
Manufacturer: BD Biosciences 566300
The SB/199 monoclonal antibody specifically binds to mouse CD127, the 65-75 kDa type-I transmembrane protein IL-7Rα. The high affinity IL-7 receptor complex is composed of at least two transmembrane proteins, IL-7Rα and CD132, the commonγ chain. CD127 has some sequence homology to the cytokine receptor superfamily (also known as the hematopoietin receptor superfamily). Micelacking CD127 display profoundly impaired development of the B and T lymphoid cell lineages, but display no obvious non-lymphoid abnormalities. IL-7Rα is expressed on common lymphoid progenitors and early stages of B lineage development in the bone marrow, on the earliest thymocyte progenitors, on CD4-CD8- double-negative and CD4+ and CD8+ single-positive thymocytes, and on most peripheral T lymphocytes. Intestinal intraepithelial lymphocytes with low-densityγδ TCR upregulate CD127 expression in response to IL-2, which may be secreted by neighboringαβ TCR-bearing T cells.The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. With an Ex Max of 407nm and Em Max at 421nm, BD Horizon™ BV421can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter). BD Horizon™ BV421conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue™ conjugates.BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences. Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates. Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.
|Aqueous buffered solution containing BSA and ≤0.09% sodium azide.|
|Interleukin-7 receptor alpha chain, IL-7R alpha, IL-7RA, IL-7Rα, Il7r|
|BALB/c mouse pre-B cell line 1A9|
|The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ BV421 under optimum conditions, and unconjugated antibody and free BD Horizon™ BV421 were|
|Brilliant Violet 421|
|Rat IgG2b, κ|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
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