CD127 Rat anti-Mouse, NovaFluor Red 710, Clone: A7R34, Invitrogen™
Rat Monoclonal Antibody
The A7R34 monoclonal antibody reacts with mouse CD127, the high affinity alpha subunit of the mouse IL-7 receptor. IL-7 receptor alpha chain is expressed by immature B cells in the bone marrow, double-negative (CD4-CD8-), single-positive (CD4+ and CD8+), but not double-positive (CD4+CD8+) thymocytes. In the periphery, mature T cells express CD127 at low level. A7R34 inhibits binding of IL-7 to its receptor and has been used in in vivo and in vitro studies to elucidate the role of IL-7 in T and B cell development and activation. Binding of A7R34 blocks the binding of SB/199, another antibody which recognizes mouse CD127. Applications Reported: The A7R34 antibody has been reported for use in flow cytometric analysis. Applications Tested: The A7R34 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 1.0 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Each NovaFluor conjugate or kit is shipped withCellBlox Blocking Buffer. Use this buffer whenever staining with NovaFluor conjugates, including single color compensation controls using cells. Use 5 uL of CellBlox Blocking Buffer per stained cell sample containing 10^3 to 10^8 cells. Excitation: 639 nm; Emission: 710 nm; Laser: 633-640 nm (Red)CD127 Monoclonal antibody specifically detects CD127 in Mouse samples. It is validated for Flow Cytometry
|PBS with 0.09% sodium azide; pH 7.2|
|CD127; CD127 antigen; CDW127; IL 7 receptor; IL 7R a; IL 7R subunit alpha; IL 7R a; IL 7Ra; IL 7Ra; IL7 receptor; IL-7 receptor alpha chain; IL7 receptor subunit alpha; IL-7 receptor subunit alpha; IL7R; IL-7R; IL7R a; IL7R alpha; IL7R subunit alpha; IL-7R subunit alpha; IL7R a; IL7RA; IL-7RA; IL-7Ralpha; IL-7R-alpha; IL7Ra; ILRA; interleukin 7 receptor; interleukin 7 receptor alpha chain; interleukin 7 receptor isoform H5-6; Interleukin7 receptor subunit alpha; interleukin-7 receptor subunit alpha; MGC107557; sCD127; soluble IL 7 receptor; soluble IL 7R|
|4° C, store in dark, DO NOT FREEZE!|
|NovaFluor Red 710|
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