CD133, Clone: 13A4,Millipore™ (Chemicon™)

Manufacturer: EMD Millipore MAB4310DEL

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Catalog No. 50-175-499

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    Specifications

    Antigen CD133
    Concentration 1mg/mL
    Conjugate Unlabeled
    Cross Reactivity Mouse
    Format Semi-purified
    Host Species Rat
    Immunogen Telencephali of ten 12-day-old NMRI mouse embryos (E12, approx. 1mg protein), which consist mostly of neuroepithelium, were homogenized in 1mL PBS, mixed with crushed nitrocellulose filter (1cm2 in 0.5mL PBS) as adjuvant, and injected i.p. into a LouXSD rat. {Weigmann et al, PNAS 1997}
    Isotype IgG1 κ
    Quantity 100μg
    Regulatory Status RUO
    Research Discipline Stem Cell Biology
    Primary or Secondary Primary
    Monoclonal or Polyclonal Monoclonal

    Prominin is a novel plasma membrane protein with an N-terminal extracellular domain, five transmembrane segments flanking two short cytoplasmic loops and two large glycosylated extracellular domains, and a cytoplasmic C-terminal domain. DNA sequences from Caenorhabditis elegans predict the existence of a protein with the same features, suggesting that prominin is conserved between vertebrates and invertebrates. Prominin is found not only in the neuroepithelium but also in various other epithelia of the mouse embryo. In the adult mouse, prominin has been detected in the brain ependymal layer, and in kidney tubules. In these epithelia, prominin is specific to the apical surface, where it is selectively associated with microvilli and microvilli-related structures. Remarkably, upon expression in CHO cells, prominin is preferentially localized to plasma membrane protrusions such as filopodia, lamellipodia, and microspikes. These observations imply that prominin contains information to be targeted to, and/or retained in, plasma membrane protrusions rather than the planar cell surface. Moreover, results show that the mechanisms underlying targeting of membrane proteins to microvilli of epithelial cells and to plasma membrane protrusions of non-epithelial cells are highly related. {Weigmann, A et al.1997}. Also see {http://www.ncbi.nlm.nih.gov/prow/guide/438375806_g.htm} for more information on CD133 and prominin expression.

    Fargeas, Christine A, et al(2003). AC133 antigen, CD133, prominin-1, prominin-2, etc.: prominin family gene products in need of a rational nomenclature.Stem Cells 21: 506-8

    Electron Microscopy, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blotting

  • Description & Specifications

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