This 5G11 monoclonal antibody recognizes human CD137 ligand also known as 4-1BB ligand, TNFSF9, or CDw137L. CD137 ligand is a member of the TNF superfamily, and is a type II membrane protein. It is a co-stimulatory molecule that is expressed on antigen presenting cells (DCs, monocytes/macrophages, B cells) and is upregulated upon activation of these cells. Its receptor, CD137 (4-1BB) is found on a variety of cells, including inflamed endothelial cells, where it enhances extravasation of CD137 ligand-expressing monocytes. Activated T cells also express CD137, and engagement with CD137 ligand enhances T cell proliferation, IL-2 secretion, survival, and cytotoxic activity. CD137 ligand is also expressed on a majority of B-cell lymphomas, including mantle cell lymphoma, follicular lymphoma, and diffuse large B-cell lymphoma (but not on Hodgkin lymphoma and T-cell lymphoma). This 5G11 antibody has been tested by immunohistochemical staining of formalin-fixed paraffin embedded human tissue using low pH antigen retreival and can be used at less than or equal to 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This transmembrane cytokine is a bidirectional signal transducer that acts as a ligand for TNFRSF9/4-1BB, which is a costimulatory receptor molecule in T lymphocytes. This cytokine and its receptor are involved in the antigen presentation process and in the generation of cytotoxic T cells. The receptor TNFRSF9/4-1BB is absent from resting T lymphocytes but rapidly expressed upon antigenic stimulation. The ligand encoded by this gene, TNFSF9/4-1BBL, has been shown to reactivate anergic T lymphocytes in addition to promoting T lymphocyte proliferation. This cytokine has also been shown to be required for the optimal CD8 responses in CD8 T cells. This cytokine is expressed in carcinoma cell lines, and is thought to be involved in T cell-tumor cell interaction.
|CD137 Ligand (4-1BB Ligand)|
|4° C, do not freeze|
|PBS with 0.09% sodium azide|
For Research Use Only
The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.
Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.Ok