CD140b (PDGFRB) Monoclonal Antibody (APB5), Alexa Fluor™ 488, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Supplier:  LIFE TECHNOLOGIES 53140282

Catalog No. 53-140-282

Description

Description: The APB5 monoclonal antibody reacts with the mouse CD140b molecule, the beta chain of the platelet derived growth factor receptor (PDGF receptor). PDGFRb is a receptor tyrosine kinase that forms dimers on the surface upon ligand binding and phosphorylates substrates. Dimers of PDGFR consist of either homodimers of alpha/alpha, beta/beta, or heterodimers of alpha/beta and serve as a substrate for its kinase activity. CD140b is expressed by embryonic tissues and mesenchymal-derived cells of the adult mouse tissues. The PDGFR beta chain is reported to play a significant role in formation of fibrous atherosclerotic lesions. Applications Reported: This APB5 antibody has been reported for use in flow cytometric analysis. Applications Tested: The APB5 antibody has been tested by flow cytometric analysis of NIH/3T3 cells. This may be used at less than or equal to 1.0 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser

CD140b (PDGFRB) Monoclonal antibody specifically detects CD140b (PDGFRB) in Mouse samples. It is validated for Flow Cytometry.

Specifications

• Antigen: CD140b (PDGFRB)
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P05622
• Gene Symbols: Pdgfrb
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 18596
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: APB5
• Conjugate: Alexa Fluor 488
• Gene: Pdgfrb
• Gene Alias: AI528809; Beta platelet-derived growth factor receptor; beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; CD140B; CD140b antigen; IBGC4; IMF1; JTK12; KOGS; PDGF beta chain; PDGF Receptor beta; Pdgfr; Pdgfr1; PDGFR-1; PDGFRB; PDGF-R-beta; PDGFR-beta; PENTT; platelet derived growth factor receptor beta; platelet derived growth factor receptor, beta polypeptide; platelet-derived growth factor receptor 1; Platelet-derived growth factor receptor beta; platelet-derived growth factor receptor beta variant 1; Platelet-derived growth factor receptor, beta; platelet-derived growth factor receptor, beta polypeptide
• Host Species: Rat
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2a κ