CD144 (VE-cadherin) Monoclonal Antibody (eBioBV14 (BV14)), eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Supplier:  Invitrogen 14144285

Catalog No. 50-128-94

Description

Description: The BV14 monoclonal antibody reacts with mouse VE-Cadherin (CD144). VE-Cadherin is a 120 kDa member of the type II Cadherin family, characterized by the presence of 5 extracellular cadherin domains (ECD), and anchored to the actin cytoskeleton through their cytoplasmic tail. VE-Cadherin mediates homophilic adhesion between neighbouring endothelial cells and is localized within specialized structures at cell-cell contacts, called adherens junctions. VE-Cadherin is expressed constitutively throughout the entire vasculature, and is required for numerous endothelial cell functions including migration, survival, contact-dependent growth inhibition and endothelial cell assembly into tubular structures. Furthermore, it is thought that VE-Cadherin+CD45- cells from the yolk sac or aorta-gonad-mesonephros (AGM)+ have the potential to give rise to hematopoietic cells. Cross-blocking experiments suggest that BV14 recognizes a different epitope than another mouse VE-Cadherin monoclonal antibody, BV13. Applications Reported: This eBioBV14 (BV14) antibody has been reported for use in immunoprecipitation, immunoblotting (WB), and immunohistology staining of frozen tissue sections. Intravenous injection of BV14 has been shown to induce a concentration- and time-dependent increase in vascular permeability in the heart and lungs, however BV13 was show to be more potent in this activity. (Please use Functional Grade purified eBioBV13 (BV13), cat. 16-1441, in functional assays.).

VE-cadherin is a member of the cadherin superfamily that is located in a six-cadherin cluster in a region on the long arm of chromosome 16 and is involved in loss of heterozygosity events in breast and prostate cancer. VE-cadherin protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, VE-cadherin may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but the full length sequence of VE-cadherin has not been determined.

Specifications

• Antigen: CD144 (VE-cadherin)
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P55284
• Gene Symbols: CDH5
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 12562
• Content And Storage: 4° C
• Form: Liquid

• Applications: Functional Assay, Immunohistochemistry (Frozen), Immunoprecipitation, Western Blot
• Clone: eBioBV14 (BV14)
• Conjugate: Unconjugated
• Gene: CDH5
• Gene Alias: 7B4; 7B4 antigen; 7B4/cadherin-5; AA408225; cadherin 5; cadherin 5, type 2 (vascular endothelium); cadherin 5, type 2, VE-cadherin (vascular endothelium); cadherin 5, type 2, VE-cadherin (vascular epithelium); cadherin-5; cadherin-5; cd144 antigen; VE cadherin; Cd144; cd144 antigen; CDH5; cell-cell adhesion protein; endothelial-specific cadherin; vascular endothelial cadherin; vascular endothelial cadherin precursor; Vec; VEcad; VE-Cad; VE-cadherin; VECD
• Host Species: Rat
• Quantity: 500 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2b

For Research Use Only.