Description: The BV14 monoclonal antibody reacts with mouse VE-Cadherin (CD144). VE-Cadherin is a 120 kDa member of the type II Cadherin family, characterized by the presence of 5 extracellular cadherin domains (ECD), and anchored to the actin cytoskeleton through their cytoplasmic tail. VE-Cadherin mediates homophilic adhesion between neighbouring endothelial cells and is localized within specialized structures at cell-cell contacts, called adherens junctions. VE-Cadherin is expressed constitutively throughout the entire vasculature, and is required for numerous endothelial cell functions including migration, survival, contact-dependent growth inhibition and endothelial cell assembly into tubular structures. Furthermore, it is thought that VE-Cadherin+CD45- cells from the yolk sac or aorta-gonad-mesonephros (AGM)+ have the potential to give rise to hematopoietic cells. Cross-blocking experiments suggest that BV14 recognizes a different epitope than another mouse VE-Cadherin monoclonal antibody, BV13. Applications Reported: This eBioBV14 (BV14) antibody has been reported for use in immunoprecipitation, immunoblotting (WB), and immunohistology staining of frozen tissue sections. Intravenous injection of BV14 has been shown to induce a concentration- and time-dependent increase in vascular permeability in the heart and lungs, however BV13 was show to be more potent in this activity. (Please use Functional Grade purified eBioBV13 (BV13), cat. 16-1441, in functional assays.).Applications Tested: This eBioBV14 (BV14) antibody has been tested by western blot analysis of bEnd.3 cell lysates, and can be used in western blotting at a starting concentration of 2 µg/mL. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered. This gene is a classical cadherin from the cadherin superfamily and is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but its full length sequence has not been determined.
|Functional Assay, Immunohistochemistry (Frozen), Immunoprecipitation, Western Blot|
|PBS with 0.09% sodium azide; pH 7.2|
For Research Use Only.
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