CD146 Monoclonal Antibody (P1H12), eFluor™ 660, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Invitrogen 50146942

Catalog No. 50-112-4307

Description

Description: The monoclonal antibody P1H12 recognizes CD146 also known as MUC18, s-endo, Endo-CAM and Mel-CAM, which is a member of the Ig superfamily of proteins. The expression of CD146 is found on endothelial cells, bone marrow fibroblasts and some tumors (especially melanoma). Recently mesenchymal stromal cells and endometrial stromal cells have also been shown to express CD146. The presence of CD146 on circulating blood cells have been confined to a subset of T cells rather than circulating endothelial cells, as expression of other endothelial markers (CD31 and CD51/61) is negative. Expression can be found on activated lymphocytes. The protein is heavily glycosylated with more than 50% of the mass from carbohydrates. The antibody P1H12 has been reported to crossreact to mouse, rabbit, canine, but not rat. Applications Reported: This P1H12 antibody has been reported for use in flow cytometric analysis. Applications Tested: This P1H12 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor™ 660 is a replacement for Alexa Fluor™ 647.

CD146 Monoclonal antibody specifically detects CD146 in Canine, Human, Mouse, Rabbit samples. It is validated for Flow Cytometry.

Specifications

• Antigen: CD146
• Classification: Monoclonal
• Concentration: 5 μL/Test
• Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P43121, Q8R2Y2
• Gene Symbols: MCAM
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 100342848, 4162, 489368, 84004
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: P1H12
• Conjugate: eFluor 660
• Gene: MCAM
• Gene Alias: 1-gicerin; AV025631; CD146; CD149; Cell surface glycoprotein MUC18; cell surface glycoprotein P1H12; Gicerin; HEMCAM; l-gicerin; l-gicerin precursor; l-gicerin protein; LOW QUALITY PROTEIN: cell surface glycoprotein MUC18; MCAM; melanoma adhesion molecule; melanoma cell adhesion molecule; Melanoma-associated antigen A32; melanoma-associated antigen MUC18; Muc18; mucin-18; s-endo; S-endo 1 endothelial-associated antigen; s-gicerin; s-gicerin precursor
• Host Species: Mouse
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Target Species: Canine, Human, Mouse, Rabbit
• Product Type: Antibody
• Isotype: IgG1 κ

For Research Use Only.