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CD15 Mouse anti-Human, Biotin, Clone: HI98, eBioscience™ Non-distribution product as customer accommodation.

Mouse Monoclonal Antibody

Manufacturer:  Invitrogen 13015982

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Catalog No. 50-114-46


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Description

Description

Description: The HI98 monoclonal antibody reacts with human CD15, Lewis X. This 3-fucosyl-N-acetyllactosamine carbohydrate moiety is expressed by granulocytes. Monocytes express this structure at varying degree while lymphocytes are negative. Applications Reported: The HI98 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen and FFPE tissue sections (IHC-F, IHC-P). Applications Tested: The HI98 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 µm post-manufacturing filtered.

CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determit (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin´s disease.
Specifications

Specifications

CD15
Monoclonal
0.5 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
stage-specific embryonic antigen 1; CD15; SSEA-1; SSEA1; ELFT; FCT3A; FUC-TIV; FUTIV; LeX
IgM, kappa
100 μg
4° C, store in dark, DO NOT FREEZE!
Primary
Human
Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)
HI98
Biotin
Liquid
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
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