CD15 Monoclonal Antibody (HI98), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Invitrogen 46015942

Catalog No. 50-159-24

Description

Description: The HI98 monoclonal antibody reacts with human CD15, Lewis X. This 3-fucosyl-N-acetyllactosamine carbohydrate moiety is expressed by granulocytes. Monocytes express this structure at varying degree while lymphocytes are negative. Applications Reported: This HI98 antibody has been reported for use in flow cytometric analysis. Applications Tested: This HI98 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation.

CD15 Monoclonal antibody specifically detects CD15 in Human samples. It is validated for Flow Cytometry.

Specifications

• Antigen: CD15
• Classification: Monoclonal
• Concentration: 5 μL/Test
• Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P22083
• Gene Symbols: FUT4
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 2526
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: HI98
• Conjugate: PerCP-eFluor 710
• Gene: FUT4
• Gene Alias: stage-specific embryonic antigen 1; CD15; SSEA-1; SSEA1; ELFT; FCT3A; FUC-TIV; FUTIV; LeX
• Host Species: Mouse
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgM κ

For Research Use Only.