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CD152 (CTLA-4) Syrian Hamster anti-Mouse, Functional Grade, Clone: 9H10, eBioscience™ Non-distribution product as customer accommodation.

Syrian Hamster Monoclonal Antibody

$97.00 - $301.00

Specifications

Antigen CD152 (CTLA-4)
Clone 9H10
Target Species Mouse
Conjugate Functional Grade
Isotype IgG
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Products 3
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
50-144-75 Non-distribution product as customer accommodation.
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Invitrogen
16152181
50 μg Each for $97.00
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50-144-76 Non-distribution product as customer accommodation.
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Invitrogen
16152182
100 μg Each for $172.00
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50-144-77 Non-distribution product as customer accommodation.
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Invitrogen
16152185
500 μg Each for $301.00
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Description

Description

Description: The 9H10 monoclonal antibody reacts with mouse CD152, also known as the cytotoxic T lymphocyte antigen-4 (CTLA-4). CTLA-4, a protein with structural similarities to CD28, is expressed on activated T cells and binds the B7 family members, CD80 (B7-1) and CD86 (B7-2), with higher affinity than CD28 does. CTLA-4 and CD28 appear to deliver opposing signals to T cells: while CD28 is a potent costimulator, CTLA-4 restricts the progression of T cells to an activated state by inhibiting IL-2 secretion and cellular proliferation. The cytoplasmic portion of CTLA-4 contains ER retention motifs, resulting in a large proportion of newly synthesized CTLA-4 in response to TCR signaling to be localized intracellularly. Applications Reported: The 9H10 antibody has been reported for use in flow cytometric analysis. It has also been reported for use in in vitro functional studies. Applications Tested: The 9H10 antibody has been tested by flow cytometric analysis of resting and Con A-stimulated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Furthermore, due to the intracellular localization of a large portion of CTLA-4, for complete detection it may be necessary to assess intracellular expression, in addition to surface expression of CTLA-4. Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay. Storage and handling: Use in a sterile environment. Filtration: 0.2 µm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC. CTLA4 (Cytotoxic T-Lymphocyte Antigen 4) is a CD28-family receptor expressed on mainly CD4+ T cells. It binds the same ligands as CD28 (CD80 and CD86 on B cells and dendritic cells), but with higher affinity than CD28. However, in contrast to CD28 which enhances cell function when bound at the same time as the T cell receptor, CTLA4 inhbits the T cell and prevents it from functioning. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function.
Specifications

Specifications

CD152 (CTLA-4)
Mouse
IgG
Monoclonal
Liquid
Antibody
4° C
9H10
Functional Grade
Flow Cytometry, Functional Assay
Primary
Monoclonal
PBS with no preservative; pH 7.2
RUO

For Research Use Only.

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