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CD154 (CD40 Ligand) Monoclonal Antibody (MR1), APC, eBioscience™, Invitrogen™
Armenian Hamster Monoclonal Antibody
Supplier: Invitrogen 17154182
Description
Description: The MR1 monoclonal antibody reacts with mouse CD154, a 39 kDa transmembrane glycoprotein also known as CD40 ligand (CD40L). CD154 is expressed transiently by activated T cells. Through its binding to CD40 on antigen presenting cells (APC) including B cells, monocytes/macrophages, and dendritic cells, it serves a crucial function in T cell-APC cognate interaction. CD154-interaction with CD40 transduces signals for T-dependent B cell activation and induces B cells to enter the cell cycle. For staining for flow cytometric analysis, it is important to stimulate enriched T cells or enriched CD4 cells (using depletion strategy) prior to staining with MR1. Applications Reported: This MR1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This MR1 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.
CD154 (CD40 Ligand) Monoclonal antibody specifically detects CD154 (CD40 Ligand) in Mouse samples. It is validated for Flow Cytometry.Specifications
CD154 (CD40 Ligand) | |
Monoclonal | |
0.2 mg/mL | |
PBS with 0.09% sodium azide; pH 7.2 | |
P27548 | |
CD40LG | |
Affinity chromatography | |
RUO | |
21947 | |
4° C, store in dark, DO NOT FREEZE! | |
Liquid |
Flow Cytometry | |
MR1 | |
APC | |
CD40LG | |
CD154; CD40 antigen ligand; CD40 ligand; CD40 ligand CD154; CD40 ligand, membrane form; CD40 ligand, soluble form; CD40L; CD40-L; Cd40lg; gp39; hCD40L; H-CD-40-L; HIGM1; IGM; IMD3; Ly62; Ly-62; M-CD-40-L; RP23-153G22.3; T-B cell-activating molecule; T-BAM; T-cell antigen Gp39; TNF-related activation protein; TNFSF5; TRAP; tumor necrosis factor (ligand) superfamily member 5; tumor necrosis factor (ligand) superfamily, member 5; tumor necrosis factor ligand superfamily member 5 | |
Armenian Hamster | |
100 μg | |
Primary | |
Mouse | |
Antibody | |
IgG |
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