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CD16/CD32 Rat anti-Mouse, Clone: 93, eBioscience™ Non-distribution product as customer accommodation.

Rat Monoclonal Antibody

$62.00 - $423.00

Specifications

Antigen CD16/CD32
Clone 93
Target Species Mouse
Conjugate Unconjugated
Isotype IgG2a, lambda
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Products 4
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
50-112-9393 Non-distribution product as customer accommodation.
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Invitrogen
14016181
50 μg Each for $62.00
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50-112-9525 Non-distribution product as customer accommodation.
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Invitrogen
14016182
100 μg Each for $101.00
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50-124-40 Non-distribution product as customer accommodation.
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Invitrogen
14016185
500 μg Each for $239.00
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50-112-9520 Non-distribution product as customer accommodation.
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Invitrogen
14016186
1 mg Each for $423.00
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Description

Description

Description: The 93 monoclonal antibody reacts with an epitope shared by mouse CD16 and CD32. CD16 (Fc gamma III Receptor) and CD32 (Fc gamma II Receptor) are the low affinity receptors for the mouse IgG Fc portion and are expressed by B cells, monocyte/macrophages, NK cells, and neutrophils. Applications Reported: The 93 antibody has been reported for use in flow cytometric analysis. 93 has also been reported in blocking of Fc-mediated reactions in functional studies. For blocking of Fc receptors in flow cytometric analysis, pre-incubate the cells with 0.5-1 µg of purified anti-CD16/CD32 per million cells for 5-10 minutes on ice prior to staining. (Please use Functional Grade purified 93, cat. 16-0161, in functional assays.). Applications Tested: The 93 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.

CD16 is a 50-65 kDa cell surface molecule that exists in two forms - a transmembranous form expressed by natural killer (NK) cells, and some T cells and a phosphatidylinositol linked form expressed by granulocytes. CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC (antibody dependent cell mediated cytotoxicity) by NK cells. IN comparison, CD32 is a 40 kD glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII), and mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets. Further, CD32 is involved in the phagocytosis of immune complexes, the regulation of antibody production by B-cells, and variations of the CD32 gene may increase vulnerability to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms of CD32 have been found.
Specifications

Specifications

CD16/CD32
Mouse
IgG2a, lambda
Monoclonal
Liquid
Antibody
4° C
93
Unconjugated
Flow Cytometry, Functional Assay, Neutralization
Primary
Monoclonal
PBS with 0.09% sodium azide; pH 7.2
RUO

For Research Use Only.

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