CD16/CD32 Rat anti-Mouse, Functional Grade, Clone: 93, eBioscience
Rat Monoclonal Antibody
Manufacturer: Invitrogen 16016182
Description: The 93 monoclonal antibody reacts with an epitope shared by mouse CD16 and CD32. CD16 (Fc gamma III Receptor) and CD32 (Fc gamma II Receptor) are the low affinity receptors for the mouse IgG Fc portion and are expressed by B cells, monocyte/macrophages, NK cells, and neutrophils. Applications Reported: The 93 antibody has been reported for use in flow cytometric analysis. 93 has also been reported in blocking of Fc-mediated reactions in functional studies. Applications Tested: The 93 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay. Storage and handling: Use in a sterile environment. Filtration: 0.2 µm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.CD16 is a 50-65 kDa cell surface molecule that exists in two forms - a transmembranous form expressed by natural killer (NK) cells, and some T cells and a phosphatidylinositol linked form expressed by granulocytes. CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC (antibody dependent cell mediated cytotoxicity) by NK cells. IN comparison, CD32 is a 40 kD glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII), and mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets. Further, CD32 is involved in the phagocytosis of immune complexes, the regulation of antibody production by B-cells, and variations of the CD32 gene may increase vulnerability to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms of CD32 have been found.
|PBS with no preservative; pH 7.2|
|FCGR3, FCGR3A, FCGR3B, CD16A, CD16B, Fc gamma Receptor III, Fc gamma Receptor 3, RP11-5K23.1, FCGR3, FCGRIII, FCR-10, FCRIII, FCRIIIA, FCGR2, CD32, Fc gamma Receptor II, CD32 receptor 2, Fc gamma Receptor 2, FCGR2A, FCGR2B, FCGR2C, Low affinity immunoglobulin gamma Fc region receptor II-b, CDw32, Fc-gamma RII-b, Fc-gamma-RIIb, FcRII-b, FCG2, Low affinity immunoglobulin gamma Fc region receptor II-c, Fc-gamma RII-c, Fc-gamma-RIIc, FcRII-c, FGFR2B|
|Flow Cytometry, Functional Assay, Neutralization|
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