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CD161, PerCP-eFluor™ 710, clone: 10/78, eBioscience™ Non-distribution product as customer accommodation.

Mouse Monoclonal Antibody

Manufacturer:  Invitrogen 46161082

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Catalog No. 50-113-0785


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Description

Description

Plays an inhibitory role on natural killer (NK) cells cytotoxicity. Activation results in specific acid sphingomyelinase/SMPD1 stimulation with subsequent marked elevation of intracellular ceramide. Activation also leads to AKT1/PKB and RPS6KA1/RSK1 kinases stimulation as well as markedly enhanced T-cell proliferation induced by anti-CD3. Acts as a lectin that binds to the terminal carbohydrate Gal-alpha(1,3)Gal epitope as well as to the N-acetyllactosamine epitope. Binds also to CLEC2D/LLT1 as a ligand and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells. {ECO:0000269; PubMed:16455998, ECO:0000269; PubMed:16925668, ECO:0000269; PubMed:8077657}.

Description: This 10/78 monoclonal antibody recognizes rat CD161a, which is also known as NKR-P1A. A member of the NKR-P1 family of type II transmembrane C-type lectins, CD161a exists as a homodimer on all NK cells, some T cells, and monocytes. In the rat, this activating receptor is also detectable on B220+ single positive B cells. CD161a is involved in activating NK cell-mediated cytotoxicity via phosphoinositide turnover and intracellular calcium mobilization.The 10/78 monoclonal antibody has been reported to recognize both NKR-P1A and NKR-P1B.Applications Reported: This 10/78 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 10/78 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PerCP-eFluor™ 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative. Our testing indicates that PerCP-eFluor™ 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2µm post-manufacturing filtered.
Specifications

Specifications

CD161
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Q0ZUP0
Ly55a, NKR-P1A, NKRP12, Nkrp1-a, Nkrp1a
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Rat
Flow Cytometry
.10/78
PerCP-eFluor 710
Klrb1
Liquid
Klrb1
IgG1, kappa, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
689817
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