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CD161a Rat anti-Mouse, Brilliant Violet 421, Clone: 3B3, BD

Rat Monoclonal Antibody

Manufacturer:  BD Biosciences 566306

Catalog No. BDB566306

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The 3B3 monoclonal antibody specifically recognizes C57BL/6 mouse CD161 antigen-like family member A (CD161a). CD161a is also known as, Natural killer cell surface protein NKR-P1A (Nkrp1a), or Lymphocyte antigen 55A (Ly55a), and is encoded by Klrb1a /(Killer cell lectin-like receptor subfamily B member 1A). CD161a is expressed on natural killer (NK) cells, and some activated T cells. CD161a may regulate natural killer (NK) cell-mediated cytotoxicity.

The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates. BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences. Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates. Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.


Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Klrb1a, Nkrp1a, NKR P1A, Nkrp1-a, Ly55a, ly-55A, NKR-P1.7, NKRP12, NKR-P1 2
Mouse NKRP1A Transfected Cell Line
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ BV421 under optimum conditions, and unconjugated antibody and free BD Horizon™ BV421 were
Brilliant Violet 421
Affinity Purified
Rat PVG, also known as HO, Black Hooded IgG2a, κ
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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