CD161b Rat anti-Mouse, Alexa Fluor 647, Clone: 2D9, BD
Rat Monoclonal Antibody
Manufacturer: BD Biosciences 566307
The 2D9 monoclonal antibody recognizes C57BL/6 mouse Natural killer cell receptor P1D (NKR-P1D) which is also known as NKR-P1B[B6], or CD161b. CD161b is encoded by Klrb1b (killer cell lectin-like receptor subfamily B member 1B). CD161b is expressed by a functionally distinct subset of natural killer (NK) cells that express higher levels of cytotoxicity and interferon- γ (IFN- γ ) than their CD161b-low or -negative counterparts. CD161b is a type II transmembrane glycoprotein that contains an immunoreceptor tyrosine-based inhibitory motif in its cytoplasmic domain. CD161b binds to C-type lectin related protein b (Clr-b) and may play a role in regulating NK cell responses. The 2D9 antibody has slight crossreactivity with cells transfected with Klrb1a /NKRP1A, Klrb1c /NKRP1C, or Klrb1f /NKRP1FAlexa Fluor™ 647 conjugates are highly photostable and remain fluorescent over a broad pH range. The excitation and emission maxima are nearly identical to those of APC. However, APC tends to be brighter while Alexa Fluor™ 647 is more optimal for intracellular applications. This fluorochrome exhibits uncommon photostability, making it an ideal choice for use in fluorescence microscopy. Due to nearly identical excitation and emission properties but different spillover characteristics, APC and Alexa Fluor™ 647 cannot be used simultaneously.
|Aqueous buffered solution containing ≤0.09% sodium azide.|
|CD161b-, CD161d, Nkrp1b, Nkrp1d, Lymphocyte antigen 55d, Ly55d|
|Mouse NKRP1D Transfected Cell Line|
|The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor™ 647 under optimum conditions, and unreacted Alexa Fluor™ 647 was removed.|
|Alexa Fluor 647|
|Rat PVG, also known as HO, Black Hooded IgG2a, λ|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
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