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CD178 (Fas Ligand) Mouse anti-Human, PE, Clone: NOK-1, eBioscience™ Non-distribution product as customer accommodation.

Mouse Monoclonal Antibody

Manufacturer:  Invitrogen 12991941

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Catalog No. 50-112-2491


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Description

Description

Description: The NOK-1 monoclonal antibody reacts with human Fas (CD95) Ligand, a 40 kDa type II transmembrane glycoprotein. FasL is a member of the TNF family and is expressed by neutrophils, monocytes, and activated T cells and NK cells. The interaction of FasL with its receptor (CD95, Fas) induces Fas-mediated killing of lymphocytes. Human FasL is cleaved from the surface by matrix metalloproteinases (MMPs), resulting in a 26 kDa soluble form. Therefore for optimal detection of surface FasL on activated peripheral blood cells, incubation of cells with an MMP inhibitor is recommended. Applications Reported: NOK-1 has been reported for use in flow cytometric analysis. Applications Tested: This NOK-1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.

Fas ligand (FasL, CD95L) is a type-II-membrane protein, whose N-terminus is in the cytoplasm and its C-terminal region extends into the extracellular space. Its receptor Fas (CD95, Apo-1) is a cell-surface-type-I-membrane protein and a member of the tumor necrosis factor (TNF) and nerve growth factor (NGF) receptor family. As a member of the TNF-cytokine family FasL induces apoptosis when interacting with its receptor Fas. FasL may exist as either membrane bound (45 kD) or soluble forms (26 kD). The soluble protein can be released from cells upon cleavage by metalloproteinases. Binding of FasL to Fas leads to oligomerization of the receptor and triggers apoptotic cell death through the interaction of other proteins. FasL is predomitly expressed in activated T-lymphocytes and natural killer (NK) cells also it is expressed in the tissues of immune-privilege sites such as testis and eye. FasL expression is also reported in various tissues as thymus, liver, ovary, lung, heart and kidney. The Fas/FasL system has been shown to play a role in a number of human diseases, for example AIDS, hepatitis or cancer. It is assumed that induction of apoptosis through FasL is predomitly involved in anti-viral immune responses.
Specifications

Specifications

CD178 (Fas Ligand)
Monoclonal
5 μL/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P48023
FasL, apoptosis antigen ligand 1, CD95 ligand, CD95L, CD178, APO1, Fas Ligand, Fas-LG
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
NOK-1
PE
FASLG
Liquid
FASLG
IgG1, kappa
25 Tests
4° C, store in dark, DO NOT FREEZE!
Primary
356
Documents
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