CD18 Monoclonal Antibody (YFC118.3), FITC, Invitrogen™

Rat Monoclonal Antibody

Supplier:  Thermo Scientific MA180644

Catalog No. PIMA180644

Description

For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL. Rat anti Human CD18 antibody, clone YFC118.3 was clustered at the Fourth International Workshop on Leucocyte Differentiation Antigen (code number N221) as recognizing the CD18 antigen.

CD18 integrin beta 2 subunit is a 90 kDa type I transmembrane protein expressed on all leucocytes. CD18 can combine with integrin molecules CD11a-c to form heterodimers at the cell surface, and these heterodimers are known to participate in the process of cell adhesion as well as cell-surface mediated signaling. CD18 forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits, and these integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1, severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis. Defects in the CD18 gene are the cause of leukocyte adhesion deficiency type I (LAD1). Two transcript variants encoding the CD18 protein have been identified.

Specifications

• Antigen: CD18
• Classification: Monoclonal
• Concentration: 0.1 mg/mL
• Gene: ITGB2
• Gene Alias: Cd18, LAD, LCAMB, Lfa1, MF17, Integrin beta-2 subunit, ITGB2, cluster designation 18
• Host Species: Rat
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 3689, 403770
• Content And Storage: 4°C or -20°C if preferred, store in dark
• Form: Liquid

• Applications: Flow Cytometry
• Clone: YFC118.3
• Conjugate: FITC
• Gene Accession No.: P05107
• Gene Symbols: ITGB2
• Immunogen: Human neutrophils.
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Target Species: Canine, Guinea Pig, Human
• Product Type: Antibody
• Isotype: IgG2b