Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

CD186 (CXCR6) Rat anti-Mouse, PerCP-eFluor™ 710, Clone: DANID2, eBioscience™ Non-distribution product as customer accommodation.

Rat Monoclonal Antibody

Manufacturer:  eBioscience 46918680

 View more versions of this product

Catalog No. 46-918-680


Add to cart

Description

Description

Description: This DANID2 monoclonal antibody recognizes mouse CD186, also known as CXCR6. This DANID2 antibody will work in flow cytometry on both native and paraformaldehyde-fixed cells. Applications Reported: This DANID2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This DANID2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 1.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product No. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product No.

00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser CD186, also known as CXCR6, is a G protein-coupled chemokine receptor reported to be one of the cellular receptors of HIV and SIV. CD186 is expressed by NKT cells, various subtypes of T cells with activated and memory phenotypes, and some plasma cells. It has been reported that mouse liver NK cells, but not naïve or splenic NK cells, express CD186. The ligand of CD186, CXCL16, is expressed by macrophages, dendritic cells and liver endothelial cells. Being highly expressed on NKT cells, CD186 is often used as a proxy marker of this cell subset.
Specifications

Specifications

CD186 (CXCR6)
Monoclonal
0.2 mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Q9EQ16
Bonzo, STRL33, TYMSTR
Rat
IgG2a, kappa
25 μg
4° C, store in dark, DO NOT FREEZE!
Primary
80901
Flow Cytometry
DANID2
PerCP-eFluor™ 710
CXCR6
Liquid
CXCR6
Mammalian cell line expressing the full form of CD186
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Documents
Provide Content Correction

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit