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CD19 Monoclonal Antibody (SJ25C1), Alexa Fluor™ 532, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Supplier: Invitrogen 58019841
Description
Description: The SJ25C1 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express this molecule. CD19, along with CD21, CD81, Leu13, and MHC class II, form a multimolecular complex that associates with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux, and proliferation of B cells. The SJ25C1 antibody and the HIB19 monoclonal antibody recognize overlapping epitopes. Applications Reported: This SJ25C1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This SJ25C1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Alexa Fluor™ 532 is excited with the Green laser (532 nm) and emits at 561 nm. This cannot be used with the Yellow-Green laser (561 nm). We recommend using a 560/14 band pass filter. Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 532 nm; Emission: 561 nm; Laser: Green Laser
CD19 Monoclonal antibody specifically detects CD19 in Human samples. It is validated for Flow Cytometry.Specifications
CD19 | |
Monoclonal | |
5 μL/Test | |
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2 | |
P15391 | |
Cd19 | |
Affinity chromatography | |
RUO | |
930 | |
4° C, store in dark, DO NOT FREEZE! | |
Liquid |
Flow Cytometry | |
SJ25C1 | |
Alexa Fluor 532 | |
Cd19 | |
AW495831; B4; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; Cd19; CD19 antigen; CD19 molecule; CVID3; differentiation antigen CD19; Leu-12; T-cell surface antigen Leu-12 | |
Mouse | |
25 Tests | |
Primary | |
Human | |
Antibody | |
IgG1 κ |
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For Research Use Only.