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CD19 Rat anti-Mouse, NovaFluor™ Blue 610-30S, Clone: eBio1D3 (1D3), Invitrogen™

Rat Monoclonal Antibody

Manufacturer:  LIFE TECHNOLOGIES M004T02B05

Encompass

Catalog No. M004T02B05


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Description

Description

The eBio1D3 (1D3) monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, MHC class II, and CD19 form a multimolecular complex that associates with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells.
Applications Reported: This eBio1D3 (1D3) antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eBio1D3 (1D3) antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.4 μg per test. A test is defined as the amount ( μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
When using more than one NovaFluor dye, CellBlox Monocyte and Macrophage Blocking Buffer may be used to block non-specific binding of dyes to monocytes.
Excitation: 509 nm; Emission: 614 nm; Laser: 488 nm (Blue) Laser

CD19 Monoclonal antibody specifically detects CD19 in Mouse samples. It is validated for Flow Cytometry
Specifications

Specifications

CD19
Monoclonal
0.1 mg/mL
PBS with no preservative; pH 7.2
P25918
AW495831; B4; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; Cd19; CD19 antigen; CD19 molecule; CVID3; differentiation antigen CD19; Leu-12; T-cell surface antigen Leu-12
IgG2a, kappa
25 μg
4° C, store in dark, DO NOT FREEZE!
Primary
12478
Flow Cytometry
eBio1D3 (1D3)
NovaFluor Blue 610-30S
Cd19
Liquid
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
SDS
Documents
Product Certifications
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