CD199 (CCR9) Monoclonal Antibody (eBioCW-1.2 (CW-1.2)), eFluor™ 450, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Invitrogen 48199182

Catalog No. 50-246-009

Description

Description: The eBioCW-1.2 monoclonal antibody reacts with mouse CCR9 (CD199), which is the receptor for thymus-expressed chemokine (TECK). CCR9 is a member of the G protein coupled receptor (GPCR) supergene family, and is involved in trafficking of T cell progenitors within the thymus. CCR9 expression during thymocyte development commences at the double-negative (DN) 3 stage (CD4-CD8-CD25+CD44-), peaks in the double-positive (DP) stage (CD4+CD8+CD25-CD44-), and is down-regulated in commited CD4+ or CD8+ single-positive (SP) thymoyctes. CCR9-deficient mice show a mild impairment in thymoycte development. In the periphery, CCR9 is thought to be expressed on naive CD8+ T cells, but not on naive CD4+ T cells. Applications Reported: This eBioCW-1.2 (CW-1.2) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBioCW-1.2 (CW-1.2) antibody has been tested by flow cytometric analysis or mouse thymocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor™ 450 is an alternative to Pacific Blue™. eFluor™ 450 emits at 445 nm and is excited with the Violet laser (405 nm).

CD199 (CCR9) Monoclonal antibody specifically detects CD199 (CCR9) in Mouse samples. It is validated for Flow Cytometry.

Specifications

• Antigen: CD199 (CCR9)
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q9WUT7
• Gene Symbols: Ccr9
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 12769
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: eBioCW-1.2 (CW-1.2)
• Conjugate: eFluor 450
• Gene: Ccr9
• Gene Alias: A130091K22Rik; C-C chemokine receptor type 9; C-C CKR-9; C-C motif chemokine receptor 9; CC-CKR-9; Ccr9; CCR-9; CDw199; CDw199 antigen; chemokine (C-C motif) receptor 9; chemokine C-C receptor 10; Cmkbr10; G protein-coupled receptor 28; GPR28; GPR-9-6; G-protein coupled receptor 28
• Host Species: Mouse
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2a

For Research Use Only.