CD1c Monoclonal Antibody (L161), NovaFluor™ Yellow 700, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Supplier:  LIFE TECHNOLOGIES H046T02Y06

Catalog No. H046T02Y06

Description

This L161 monoclonal antibody detects CD1c, a glycoprotein that is noncovalently linked to beta-2 microglobulin on thymocytes and antigen presenting cells such as dendritic and Langerhans cells. This molecule is also expressed on some circulating and marginal zone B cells, as well as in lymph nodes and germinal centers. CD1c is involved in the presentation of lipid antigens such as microbial fatty acids to effector T cells during the adaptive immune response. Finally, alternative splicing gives rise to three different isoforms of CD1c (soluble, membrane, and cytoplasmic/soluble isoforms). Applications Tested: This L161 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5μL (0.06μg) per test Our internal testing shows that NovaFluor Yellow 700 non-specifically stains B cells in SJL mice. Non-specific staining has not been observed in BALB/c or C57BL/6 mice. Other strains have not been tested. See the Antibody Testing Data for an example of this strain-dependent difference. NovaFluor dyes are not compatible with DNA intercalating viability dyes. Do not use viability dyes such as propidium iodide, 7-actinomycin D (7-AAD) and DAPI. Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes. Each NovaFluor conjugate or kit is shipped with CellBlox Blocking Buffer. Excitation: 552 nm; Emission: 700 nm; Laser: 561 nm (Yellow) Laser

CD1c Monoclonal antibody specifically detects CD1c in Human samples. It is validated for Flow Cytometry

Specifications

• Antigen: CD1c
• Classification: Monoclonal
• Concentration: 0.05 μg/Test
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P29017
• Host Species: Mouse
• Quantity: 25 Tests
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1 κ

• Applications: Flow Cytometry
• Clone: L161
• Conjugate: NovaFluor Yellow 700
• Gene: CD1C
• Gene Alias: BDCA1; canCD1c; CD1; CD1A; CD1C; CD1C antigen, c polypeptide; CD1c molecule; cortical thymocyte antigen CD1C; differentiation antigen CD1-alpha-3; R7; RP11-101J8.3; T-cell surface glycoprotein CD1c
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 911
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid