CD1d Mouse anti-Mouse, Rat, APC, Clone: WTH-2, eBioscience
Mouse Monoclonal Antibody
Manufacturer: Invitrogen 17001380
Description: The monoclonal antibody WTH-2 reacts with mouse and rat CD1d. CD1d is a non-polymorphic MHC Class I-like molecule composed of alpha-1 and alpha-2 domains. CD1d is constitutively expressed on hematopoietic cells, with highest levels on dendritic cells, macrophages, and marginal zone B-cells. It is also expressed on non-lymphatic organs such as the liver, heart, small intestine, and pancreas. CD1d associates with beta2-microglobulin to present antigens containing a lipid or other hydrophobic moieties to invariant NKT cells, which leads to rapid Th1 or Th2 cytokine secretion. The WTH-2 antibody has been reported to have antagonist activity. Applications Reported: This WTH-2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This WTH-2 antibody has been tested by flow cytometric analysis of mouse or rat splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.CD1d is a 49 kDa heavy chain associated with Beta 2 microglobulin on cortical thymocytes. Beta 2 microglobulin independent expression of CD1d has also been demonstrated on human intestinal epithelial cells. CD1d is the sole group 2 member of the CD1 family of major histocompatibility (MHC) like glycoproteins. The CD1d gene encodes a divergent member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by the CD1d gene localizes to late endosomes and lysosomes via a tyrosine-based motif in the cytoplasmic tail. Diseases associated with CD1D include Mycobacterium Malmoense and Autoimmune Disease.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|AI747460, CD1.1, Cd1a, Cd1d, Ly-38, T-cell surface glycoprotein CD1d1, antigen-presenting glycoprotein CD1d1|
|4° C, store in dark, DO NOT FREEZE!|
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