CD20 Monoclonal Antibody (L26), eFluor™ 660, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Invitrogen 50020282

Catalog No. 50-112-4259

Description

Description: The monoclonal antibody L26 recognizes human CD20, a two transmembrane spanning protein found on precursor and mature B lymphocytes. Expression is lost upon differentiation to plasma cells. The levels of CD20 are critical in evaluating maligt B lymphocytes including Non-Hodgkin's Lymphoma, and Acute B cell, Chronic Lymphocytic and Hairy-Cell Leukemias. CD20 plays a role in B cell differentiation and activation. The structure of the protein shows a small extracellular loop with the carboxy and amino termini in the cytoplasmic compartment. The L26 antibody recognizes an epitope in the cytoplasmic domain and therefore requires permeabilization of the tissue for staining applications. Unlike many anti-CD20 antibodies that recognize the extracellular domain, the epitope recognized by L26 will not be blocked by therapeutic antibodies to CD20, like Rituximab. Applications Reported: This L26 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. Applications Tested: This L26 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tonsils using IHC Antigen Retrieval Solution - Low pH (cat. 00-4955). eFluor™ 660 is a replacement for Alexa Fluor™ 647. eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

CD20 Monoclonal antibody specifically detects CD20 in Cynomolgus Monkey, Human, Rhesus Monkey samples. It is validated for Immunocytochemistry, Immunohistochemistry, Immunohistochemistry (Paraffin), Western Blot.

Specifications

• Antigen: CD20
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P11836
• Gene Symbols: Ms4a1
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 102118491, 696843, 931
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Immunocytochemistry, Immunohistochemistry, Immunohistochemistry (Paraffin), Western Blot
• Clone: L26
• Conjugate: eFluor 660
• Gene: Ms4a1
• Gene Alias: AA960661; APY; ATOPY; B1; B-cell differentiation antigen Ly-44; B-lymphocyte antigen CD20; B-lymphocyte cell-surface antigen B1; B-lymphocyte surface antigen B1; Bp35; Cd20; CD20 antigen; CD20 cell surface protein; CD20 receptor; CVID5; EGK_06167; Fc epsilon receptor I beta chain; Fc Fragment of IgE high affinity I receptor for beta polypeptide; FCER1B; High affinity immunoglobulin epsilon receptor subunit beta; IgE Fc receptor subunit beta; IGEL; IGER; IGHER; LEU16; LEU-16; Leukocyte surface antigen Leu-16; Ly44; Ly-44; Lymphocyte antigen 44; membrane spanning 4-domains A1; membrane-spanning 4-domains subfamily A member 1; membrane-spanning 4-domains, subfamily A, member 1; membrane-spanning 4-domains, subfamily A, member 2; MGC3969; MS4A1; MS4A2; S7
• Host Species: Mouse
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Cynomolgus Monkey, Human, Rhesus Monkey
• Product Type: Antibody
• Isotype: IgG2a κ

For Research Use Only.