Description: The monoclonal antibody RCR-227 reacts with human CD201, which is also known as the endothelial protein C receptor (EPCR), a 46-kDa transmembrane protein related to class I MHC. CD201 was originally identified on vascular endothelium cells, predomitly larger blood vessels. It is also expressed on monocytes, lymphocytes, hematopoietic stem cells, neutrophils, and eosinophils. CD201 serves as a binding site for protein C, activated protein C (APC), and factor VII/VIIa. A soluble form has also been identified in the plasma. CD201 plays a role in controlling coagulation as disruption of its gene in mice caused placental thrombosis and early embryonic death. This cell surface molecule is also involved in the anti-inflammatory response. APC binding to CD201 enables PAR-1 activation that confers cytoprotective effects. In monocytes, APC binding decreases secretion of proinflammatory cytokines, chemokines, and growth factors. Finally, CD201 has been shown to inhibit lymphocyte migration. Applications Reported: This RCR-227 antibody has been reported for use in flow cytometric analysis. Applications Tested: This RCR-227 antibody has been pre-titrated and tested by flow cytometric analysis of HUVECs. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL.Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered. The protein encoded by this gene is a receptor for activated protein C, a serine protease activated by and involved in the blood coagulation pathway. The encoded protein is an N-glycosylated type I membrane protein that enhances the activation of protein C. Mutations in this gene have been associated with venous thromboembolism and myocardial infarction, as well as with late fetal loss during pregcy.
|4° C, store in dark, DO NOT FREEZE!|
|PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2|
For Research Use Only.
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