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CD209 (DC-SIGN) Rat anti-Human, APC, Clone: eB-h209, eBioscience™ Non-distribution product as customer accommodation.

Rat Monoclonal Antibody

Manufacturer:  Invitrogen 17209942

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Catalog No. 50-112-3107


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Description

Description

Description: The eB-h209 monoclonal antibody reacts with human CD209, also known as DC-SIGN, a 44 kDa type II transmembrane protein. DC-SIGN contains a C-type lectin binding domain and binds ICAM-3, ICAM-2, and HIV virus. Human dendritic cells preferentially express DC-SIGN. It has been postulated that DC-SIGN serves as a receptor for capture, trafficking, and transmission of HIV to T cells and supports primary immune response. eB-h209 was developed against a C-terminal peptide of human DC-SIGN. Applications Reported: The eB-h209 antibody has been reported for use in flow cytometric analysis. Applications Tested: This eB-h209 antibody has been pre-titrated and tested by flow cytometric analysis of cultured human dendritic cells and peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.

This gene encodes a transmembrane receptor and is often referred to as DC-SIGN because of its expression on the surface of dendritic cells and macrophages. The encoded protein is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens ranging from parasites to viruses with a large impact on public health. The protein is organized into three distinct domains: an N-terminal transmembrane domain, a tandem-repeat neck domain and C-type lectin carbohydrate recognition domain. The extracellular region consisting of the C-type lectin and neck domains has a dual function as a pathogen recognition receptor and a cell adhesion receptor by binding carbohydrate ligands on the surface of microbes and endogenous cells. The neck region is important for homo-oligomerization which allows the receptor to bind multivalent ligands with high avidity. Variations in the number of 23 amino acid repeats in the neck domain of this protein are rare but have a significant impact on ligand binding ability. This gene is closely related in terms of both sequence and function to a neighboring gene (GeneID 10332; often referred to as L-SIGN). DC-SIGN and L-SIGN differ in their ligand-binding properties and distribution. Alternative splicing results in multiple variants.
Specifications

Specifications

CD209 (DC-SIGN)
Monoclonal
5 μL/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
Q9NNX6
CD209, CDSIGN, CIRE, DC-SIGN, DC-SIGN1, Dcsign, MGC130443, SIGN-R1, SIGNR5, CD209a
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
eB-h209
APC
CD209
Liquid
CD209
IgG2a, kappa
100 Tests
4° C, store in dark, DO NOT FREEZE!
Primary
30835
Documents
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