CD218a (IL-18Ra) Rat anti-Mouse, eFluor® 450, Clone: P3TUNYA, eBioscience
Rat Monoclonal Antibody
Manufacturer: Invitrogen 48518382
Description: This P3TUNYA monoclonal antibody reacts with mouse CD218a (Interleukin 18 Receptor alpha). CD218a is one of the subunits of the heterodimeric receptor complex for the IL-18 cytokine. IL-18 is a proinflammatory cytokine and member of the IL-1 family first identified by its role in IFN gamma secretion during toxic shock. Like the other members of the family, it is synthesized as an inactive precursor which requires cleavage by caspases prior to secretion of the active form. It is secreted by activated monocytes, macrophages, epithelial cells, and keratinocytes via a non-classical pathway. IL-18 Receptor is expressed on Th1, but not naive CD4 T cells or Th2, as well as subsets of NK cells and neutrophils. The heterodimeric receptor is made up of a ligand-binding alpha chain and signal-transducing beta chain. On its own, IL-18Ra binds IL-18 with low affinity, although the affinity of this interaction increases upon forming a complex with IL-18Rb. IL-18R signaling plays a critical role in Th1 differentiation and effector function, and results in the increased expression of IFN gamma, TNF alpha, and Fas ligand from T cells and NK cells. IL-18 has also been found to synergize with IL-23 in the development and function of Th17 cells. Recently, a subset of CD8 T cells has been discovered that co-secretes IFN gamma with IL-17A and sometimes IL-22. These Tc17 cells share key transcription factors, chemokine receptors, and cytokine receptors with Th17 cells.In humans, this population has been identified by expression of CD161 and IL-18R. As a CD161 homolog has yet to be identified in mice, it has been suggested that IL-18R may be used as a surrogate for CD161 in mouse Tc17 phenotyping. Applications Reported: This P3TUNYA antibody has been reported for use in flow cytometric analysis. Applications Tested: This P3TUNYA antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser. Filtration: 0.2 µm post-manufacturing filtered. IL18R1 is a cytokine receptor that belongs to the interleukin 1 receptor family. This receptor specifically binds interleukin 18 (IL18), and is essential for IL18 mediated signal transduction. IFN-alpha and IL12 are reported to induce the expression of this receptor in NK and T cells. The gene along with four other members of the interleukin 1 receptor family, including IL1R2, IL1R1, ILRL2 (IL-1Rrp2), and IL1RL1 (T1/ST2), form a gene cluster on chromosome 2q.
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