CD22 Mouse anti-Human, PerCP-eFluor 710, Clone: eBio4KB128 (4KB128), eBioscience
Mouse Monoclonal Antibody
Manufacturer: Invitrogen 46022942
Description: The eBio4KB128 monoclonal antibody recognizes human CD22 (Siglec-2), which is a member of the siglec subgroup of the Ig superfamily. CD22 is a type I transmembrane glycoprotein composed of two polypeptide chains, CD22 alpha and CD22 beta, of 130 and 140 kDa respectively, produced by alternative splicing of the CD22 gene. CD22 is expressed at high levels on mature B cells and B cell lymphomas. The extracellular portion of CD22 contains seven Ig-like domains, some of which are capable of binding ligands with sialic acid moieties expressed on epithelial, endothelial, B and T cells. The intracellular portion of CD22 contains 6 tyrosine residues contained within immunotyrosine-based inhibitory motifs (ITIM) and immunotyrosine-based activation-like motifs, which are phosphorylated upon B-cell receptor engagement, which enables CD22 to participate in the positive and negative regulation of B-cell receptor signaling. Applications Reported: This eBio4KB128 (4KB128) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBio4KB128 (4KB128) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered. CD22 (BL-CAM) is a type 1 integral membrane glycoprotein with molecular weight of 130 to 140 kDa. CD22 is expressed in both the cytoplasm and cell membrane of B-lymphocytes. CD22 antigen appears early in B-cell lymphocyte differentiation at approximately the same stage as the CD19 antigen. Unlike other B-cell markers, CD22 membrane expression is limited to the late differentiation stages comprised between mature B cells (CD22+) and plasma cells (CD22-), and may thus prove useful in phenotyping mature leukemias. CD22 is also strongly expressed in hairy cell leukemia. CD22 preferentially binds to alpha2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B cell antigen receptor signaling. CD22 plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules. CD22 is also strongly expressed in hairy cell leukemia. CD22 is also positive in diffuse large B-cell lymphoma and nodular lymphocyte predomice Hodgkin’s lymphoma, but negative in classical Hodgkin’s lymphoma.
|PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2|
|FLJ22814, MGC130020, SIGLEC-2, SIGLEC2|
|4° C, store in dark, DO NOT FREEZE!|
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