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CD223 (LAG-3) Mouse anti-Human, Super Bright 436, Clone: 3DS223H, eBioscience™ Non-distribution product as customer accommodation.

Mouse Monoclonal Antibody

$163.00 - $345.00

Specifications

Antigen CD223 (LAG-3)
Clone 3DS223H
Target Species Human
Conjugate Super Bright 436
Isotype IgG1, kappa
View More Specs

Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
62-223-942 Non-distribution product as customer accommodation. View Documents Invitrogen
62223942
100 Tests Each for $345.00
Add to cart
 
62-223-941 Non-distribution product as customer accommodation. View Documents Invitrogen
62223941
25 Tests Each for $163.00
Add to cart
 
Description

Description

Description: This 3DS223H monoclonal antibody recognizes human CD223 also known as Lymphocyte Activation Gene 3 (LAG-3). LAG-3 is a 70-kDa surface glycoprotein belonging to the Ig superfamily with homology to CD4. LAG-3 binds to MHC class II with higher affinity than CD4 and is thought to be involved in the negative regulation of T cell activation and homeostatic proliferation. Surface expression of LAG-3 has been reported on activated T cells (including regulatory T cells) and NK cells. CD8+ T cells usually express LAG-3 at significantly higher levels than CD4+ T cells. Coexpression of LAG-3 and CD49b has been proposed to identify human and mouse Type 1 regulatory T cells (Tr1 cells). This 3DS223H antibody will recognize a formaldehyde-fixed epitope. Applications Reported: This 3DS223H antibody has been reported for use in flow cytometric analysis. Applications Tested: This 3DS223H antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent.

Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (cat. SB-4400) to minimize any non-specific polymer interactions. Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser LAG-3 is a 70-kDa surface glycoprotein belonging to the Ig superfamily with homology to CD4. LAG-3 binds to MHC class II with higher affinity than CD4 and is thought to be involved in the negative regulation of T cell activation and homeostatic proliferation. Surface expression of LAG-3 has been reported on activated T cells (including regulatory T cells) and NK cells. CD8+ T cells usually express LAG-3 at significantly higher levels than CD4+ T cells. Coexpression of LAG-3 and CD49b has been proposed to identify human and mouse Type 1 regulatory T cells (Tr1 cells).
Specifications

Specifications

CD223 (LAG-3)
Human
IgG1, kappa
Monoclonal
Liquid
Antibody
4° C, store in dark, DO NOT FREEZE!
3DS223H
Super Bright 436
Flow Cytometry
Primary
Monoclonal
PBS with BSA and 0.09% sodium azide; pH 7.2
RUO

For Research Use Only

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