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Invitrogen™ CD226/DNAM-1 Human ELISA Kit

Sandwich ELISA Kit

Supplier:  Invitrogen™ EHCD226

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Catalog No. EHCD226


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Description

Description

The Human CD226/DNAM-1 ELISA quantitates Hu CD226 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu CD226. Principle of the method The Human CD226 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

DNAM 1 is a receptor expressed by peripheral blood T lymphocytes that is involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T lymphocytes. DNAM 1 is broadly expressed on T cells, NK cells, platelets, monocytes and a subset of B cells. DNAM-1 is also expressed by a subset of CD3 positive thymocytes. This antibody is reported to inhibit T- and NK cell mediated cytotoxicity against tumour cell targets and to block TNF alpha and IFN gamma secretion by alloantigen-specific T-cells.
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Specifications

Specifications

Q15762
5 ng/mL
Solid-phase sandwich ELISA
ELISA Kit
Plasma, Serum, Supernatant
96 assays
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
10666
<12%
Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
96 Tests
Extracellular Matrix & Cell Adhesion
2°C to 8°C
Human
4 hr. 45 min.
6.14-600 ng/mL
5ng/mL to 600ng/mL
Biotin
CD Protein
This ELISA pair antibody detects human DNAM1. Other species not determined.
Human
Colorimetric Microplate Reader
DNAM-1,DNAM1,PTA1,TLiSA1
<10%
HRP
RUO
Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL
DNAM-1, DNAM1, PTA1, TLiSA1
1 hr. 20 min.
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