CD226 (DNAM-1) Monoclonal Antibody (11A8.7.4), PE-Cyanine7, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
25-226-942	Invitrogen™ 25226942	100 Tests

Description

The monoclonal antibody 11A8.7.4 reacts to DNAM-1 also known as CD226. DNAM 1 is a 65 kD receptor expressed by peripheral blood T lymphocytes that is involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T lymphocytes. DNAM 1 is broadly expressed on T cells, NK cells, platelets, monocytes and a subset of B cells. DNAM-1 is also expressed by a subset of CD3 positive thymocytes. The ligands for CD226 are the poliovirus receptor (PVR, CD155) and Poliovirus Related Receptor (PPR2, Nectin-2, CD112). CD226 is involved in cytolytic activity by interacting with integrins and activating protein tyrosine kinase Fyn. The 11A8.7.4 antibody has been shown to increase T and NK cell-mediated cytotoxicity. Applications Reported: This 11A8.7.4 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 11A8.7.4 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product No.

DNAM 1 is a receptor expressed by peripheral blood T lymphocytes that is involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T lymphocytes. DNAM 1 is broadly expressed on T cells, NK cells, platelets, monocytes and a subset of B cells. DNAM-1 is also expressed by a subset of CD3 positive thymocytes.

Specifications

• Antigen: CD226 (DNAM-1)
• Concentration: 5 μL/Test
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Gene Accession No.: Q15762
• Isotype: IgG1 κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

• Clone: 11A8.7.4
• Applications: Flow Cytometry
• Conjugate: PE-Cyanine7
• Host Species: Mouse
• Target Species: Human
• Gene ID (Entrez): 10666
• Primary or Secondary: Primary
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!