CD235a (Glycophorin A) Monoclonal Antibody (HIR2 (GA-R2)), NovaFluor™ Red 685, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Supplier:  LIFE TECHNOLOGIES H050T02R02

Catalog No. H050T02R02

Description

The HIR2 monoclonal antibody reacts with human glycophorin A, a sialoglycoprotein expressed by erythroid precursors and mature circulating red cells. Applications Reported: This HIR2 (also GA-R2) antibody has been reported for use in flow cytometric analysis. Applications Tested: This HIR2 (also GA-R2) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.015 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. NovaFluor dyes are not compatible with DNA intercalating viability dyes. Do not use viability dyes such as propidium iodide, 7-actinomycin D (7-AAD) and DAPI. Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes. Each NovaFluor conjugate or kit is shipped with CellBlox Blocking Buffer. Use this buffer whenever staining with NovaFluor conjugates, including single color compensation controls using cells. Use 5 μL of CellBlox Blocking Buffer per stained cell sample containing 10^3 to 10^8 cells. NovaFluor conjugates are based on Phiton™ technology utilizing novel nucleic acid dye structures that allow for engineered fluorescent signatures with consideration for spillover and spread impacts. Excitation: 637 nm; Emission: 685 nm; Laser: 633-640 nm (Red) Laser

CD235a (Glycophorin A) Monoclonal antibody specifically detects CD235a (Glycophorin A) in Human samples. It is validated for Flow Cytometry

Specifications

• Antigen: CD235a (Glycophorin A)
• Classification: Monoclonal
• Concentration: 0.05 μg/Test
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P02724
• Host Species: Mouse
• Quantity: 25 Tests
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2b κ

• Applications: Flow Cytometry
• Clone: HIR2 (GA-R2)
• Conjugate: NovaFluor Red 685
• Gene: GYPA
• Gene Alias: AI853584; CD235; CD235a; erythroid-lineage-specific membrane sialoglycoprotein; glycophorin A; glycophorin A (MN blood group); glycophorin A (MNS blood group); glycophorin A, GPA; glycophorin Erik; glycophorin MiI; glycophorin MiV; glycophorin SAT; glycophorin Sta type C; glycophorin-A; GPA; GPErik; GPSAT; Gypa; HGNC:4702; HGpMiV; HGpMiX; HGpMiXI; HGpSta(C); HGpStaC; Mi.V glycoprotein; Mi.V glycoprotein (24 AA); MN; MN sialoglycoprotein; MNS; PAS-2; recombinant glycophorin A-B Miltenberger-DR; sialoglycoprotein alpha
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 2993
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid